CCQM-K181
MEASURAND Copy number concentration
NOMINAL VALUE 10 × 105 μL-1
Degrees of equivalence represented by Di = (xi - xR) and its expanded uncertainty Ui at a 95 % level of confidence, both expressed in μL-1.
CCQM-K181
MEASURAND Copy number concentration
NOMINAL VALUE 10 × 101 μL-1
Degrees of equivalence represented by Di = (xi - xR) and its expanded uncertainty Ui at a 95 % level of confidence, both expressed in μL-1.
CCQM-K181
MEASURAND Copy number concentration
NOMINAL VALUE 10 × 105 μL-1
Degrees of equivalence represented by Di = (xi - xR) and its expanded uncertainty Ui at a 95 % level of confidence, both expressed in μL-1.
| LABi | Di | Uneg,i | Upos,i | |
|---|---|---|---|---|
µL-1 |
µL-1 |
µL-1 |
||
| INM (CO) | -3.24E+05 | 1.07E+05 | ||
| MIRS/NIB/FITO | -2.74E+05 | 7.00E+04 | ||
| NIMT | -2.67E+05 | 1.17E+05 | ||
| PTB | -2.56E+05 | 8.08E+04 | ||
| NIST | -2.02E+05 | 9.39E+04 | ||
| KRISS | -1.87E+05 | 1.26E+05 | ||
| VNIIM | -1.52E+05 | 1.12E+05 | ||
| UME | -1.32E+05 | 9.58E+04 | ||
| JRC | -1.17E+05 | 1.02E+05 | ||
| LGC | -1.17E+05 | 1.10E+05 | ||
| INMETRO | -1.11E+05 | 9.91E+04 | ||
| INRIM | -2.09E+04 | 1.73E+05 | ||
| NIM | -1.70E+04 | 8.49E+04 | ||
| NMIJ | 2.30E+04 | 2.28E+05 | ||
| NMIA | 2.30E+04 | 1.63E+05 |
CCQM-K181
MEASURAND Copy number concentration
NOMINAL VALUE 10 × 101 μL-1
Degrees of equivalence represented by Di = (xi - xR) and its expanded uncertainty Ui at a 95 % level of confidence, both expressed in μL-1.
| LABi | Di | Uneg,i | Upos,i | |
|---|---|---|---|---|
µL-1 |
µL-1 |
µL-1 |
||
| NIMT | -23.7 | 10.82 | ||
| VNIIM | -19.9 | 8.86 | ||
| INM (CO) | -17.1 | 16.23 | ||
| PTB | -17.1 | 10.49 | ||
| JRC | -14.0 | 7.10 | ||
| LGC | -14.0 | 9.04 | ||
| KRISS | -12.5 | 10.32 | ||
| INMETRO | -11.0 | 15.23 | ||
| INRIM | -10.7 | 9.45 | ||
| NIST | -10.2 | 10.03 | ||
| UME | -8.0 | 10.00 | ||
| NIM | 2.0 | 8.77 | ||
| NMIA | 2.0 | 16.16 | ||
| NMIJ | 11.0 | 48.37 |
| Metrology area, Sub-field | Chemistry and Biology, Nucleic Acid Analysis |
| Description | SARS-CoV-2 RNA copy number quantification |
| Time of measurements | 2022 - 2023 |
| Status | Approved for equivalence |
| Final Reports of the comparisons | |
| Measurand | Copy number concentration of single stranded RNA synthetic molecule (obtained by in vitro transcription) containing the SARS-CoV-2 E,N and partial ORF 1ab coding regions |
| Comparison type | Key Comparison |
| Consultative Committee | CCQM (Consultative Committee for Amount of Substance) |
| Conducted by | CCQM (Consultative Committee for Amount of Substance) |
| Comments | The key comparison is run in parallel with the pilot study CCQM-P227. Quantitative viral genome copy measurements are important in a number of pathogens where viral load informs treatment, such has HIV or Hepatitis C. The diagnostic response to the COVID-19 also illustrated the importance of quantitative measurements in informing the performance of tests where the presence, and not quantity, of the pathogen is clinically required. This study will provide NMIs with a route to demonstrate the core competencies to deliver measurement services of RNA copy number measurement to respond to national needs in the global response to the SARS-CoV-2 pandemic. Results published 03 February 2026 |
| Pilot institute |
NIM
National Institute of Metrology China |
| Contact person | Lianhua Dong |
| Additional |
| Pilot laboratory | |
|---|---|
| NIM |
National Institute of Metrology, China, APMP |
| INM (CO) |
Instituto Nacional de Metrología de Colombia, Colombia, SIM |
| INMETRO |
Instituto Nacional de Metrologia, Qualidade e Tecnologia, Brazil, SIM |
| INRIM |
Istituto Nazionale di Ricerca Metrologica, Italy, EURAMET |
| JRC |
Joint Research Centre, European Union, EURAMET |
| KRISS |
Korea Research Institute of Standards and Science, Korea, Republic of, APMP |
| LGC |
Laboratory of the Government Chemist, United Kingdom, EURAMET |
| MIRS/NIB/FITO |
MIRS/National Institute of Biology/Department of Biotechnology and Systems Biology, Slovenia, EURAMET |
| NIMT |
National Institute of Metrology (Thailand), Thailand, APMP |
| NIST |
National Institute of Standards and Technology, United States, SIM |
| NMIA |
National Measurement Institute, Australia, Australia, APMP |
| NMIJ AIST |
National Metrology Institute of Japan, Japan, APMP |
| PTB |
Physikalisch-Technische Bundesanstalt, Germany, EURAMET |
| UME |
TÜBITAK Ulusal Metroloji Enstitüsü, Türkiye, EURAMET |
| VNIIM |
D.I. Mendeleyev Institute for Metrology, Rosstandart, Russian Federation, COOMET |
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CCQM-K181
MEASURAND Copy number concentration
NOMINAL VALUE 10 × 105 μL-1
The key comparison reference value xR of S1 and its associated standard uncertainty uR were calculated based on result of S0 and the gravimetric dilution factors as described in page 31 of the CCQM-K181 Final report.
xR = 8.27 × 105 μL-1 uR = 2.90 × 104 μL-1
The degree of equivalence of laboratory i relative to the key comparison reference value xR is given by a pair of terms, both expressed in μL-1:
Di = (xi - xR) and its expanded uncertainty Ui at a 95 % level of confidence where Ui = 2 (ui2 + uR2)1/2
CCQM-K181
MEASURAND Copy number concentration
NOMINAL VALUE 10 × 101 μL-1
The key comparison reference value xR of S2 and its associated standard uncertainty uR were calculated based on result of S0 and the gravimetric dilution factors as described in page 31 of the CCQM-K181 Final report.
xR = 64 μL-1 uR = 3 μL-1
The degree of equivalence of laboratory i relative to the key comparison reference value xR is given by a pair of terms, both expressed in μL-1:
Di = (xi - xR) and its expanded uncertainty Ui at a 95 % level of confidence where Ui = 2 (ui2 + uR2)1/2
CCQM-K181
MEASURAND Copy number concentration
NOMINAL VALUE 10 × 105 μL-1
xi measurement value submitted by institute i, Labi
ui combined standard uncertainty of xi
| Labi | xi | ui | |
| µL-1 | µL-1 | ||
| PTB | 5.71E+05 | 2.81E+04 | |
| INMETRO | 7.16E+05 | 4.02E+04 | |
| KRISS | 6.40E+05 | 5.60E+04 | |
| NIM | 8.10E+05 | 3.10E+04 | |
| NMIJ | 8.50E+05 | 1.10E+05 | |
| JRC | 7.10E+05 | 4.20E+04 | |
| UME | 6.95E+05 | 3.81E+04 | |
| INM (CO) | 5.03E+05 | 4.51E+04 | |
| INRIM | 8.06E+05 | 8.13E+04 | |
| LGC | 7.10E+05 | 4.65E+04 | |
| NIST | 6.25E+05 | 3.69E+04 | |
| NMIA | 8.50E+05 | 7.60E+04 | |
| NIMT | 5.60E+05 | 5.10E+04 | |
| VNIIM | 6.75E+05 | 4.81E+04 | |
| MIRS/NIB/FITO | 5.53E+05 | 1.96E+04 |
CCQM-K181
MEASURAND Copy number concentration
NOMINAL VALUE 10 × 101 μL-1
xi measurement value submitted by institute i, Labi
ui combined standard uncertainty of xi
| Labi | xi | ui | |
| µL-1 | µL-1 | ||
| PTB | 46.9 | 4.3 | |
| INMETRO | 53 | 7 | |
| KRISS | 51.5 | 4.2 | |
| NIM | 66 | 3.2 | |
| NMIJ | 75 | 24 | |
| JRC | 50 | 1.9 | |
| UME | 56 | 4 | |
| INM (CO) | 46.87 | 7.54 | |
| INRIM | 53.3 | 3.65 | |
| LGC | 50 | 3.38 | |
| NIST | 53.82 | 4.02 | |
| NMIA | 66 | 7.5 | |
| NIMT | 40.3 | 4.5 | |
| VNIIM | 44.1 | 3.26 |
