| Metrology area, Sub-field | Chemistry and Biology, Nucleic Acid Analysis |
| Description | Relative quantification of genomic DNA fragments extracted from protein rich matrix |
| Time of measurements | 2021 - 2022 |
| Status | Measurements completed |
| Measurand | DNA copy number ratio of beta-actin gene copy of pork to (1) beta-actin gene copies of pork and beef (2) total myostatin gene copy DNA mass fraction of beta-actin gene mass of pork to beta-actin gene masses of pork and beef |
| Parameters | low and high DNA copy number ratio (matrix) low and high mass fraction (matrix) DNA copy number ratio (solution) |
| Transfer device | Study material 1: for protein rich matrix, autoclaved pork (represent for processed food) was mixed with autoclaved beef (DNA copy number ratio, conversion factor, mass fraction (w/w)) Study material 2: for solution sample, pork DNA solution was mixed in beef DNA solution (DNA copy number ratio). |
| Comparison type | Key Comparison |
| Consultative Committee | CCQM (Consultative Committee for Amount of Substance) |
| Conducted by | CCQM (Consultative Committee for Amount of Substance) |
| Comments | With K86 series, the quantification of the relative abundance of two DNA fragments in a matrix with high carbohydrate and high fat content has been demonstrated in plant material in previous key comparisons (K86, K86.b and K86.c). The measurement space of food matrix lacks high protein content and the DNA extraction process had not been studied for processed matrix, so, this study as a protein rich matrix model for genomic DNA quantification (pork matrix in beef matrix) will fill in this area. |
| Pilot institute |
NIMT
National Institute of Metrology (Thailand) Thailand |
| Contact person | Phattaraporn morris +66917809985 |
| Additional |
| Pilot laboratory | |
|---|---|
| NIMT |
National Institute of Metrology (Thailand), Thailand, APMP |
| BVL |
Bundesamt für Verbraucherschutz und Lebensmittelsicherheit, Germany, EURAMET |
| GL |
Government Laboratory, Hong Kong, China, APMP |
| INM (CO) |
Instituto Nacional de Metrología de Colombia, Colombia, SIM |
| INMETRO |
Instituto Nacional de Metrologia, Qualidade e Tecnologia, Brazil, SIM |
| KRISS |
Korea Research Institute of Standards and Science, Korea, Republic of, APMP |
| LGC |
Laboratory of the Government Chemist, United Kingdom, EURAMET |
| MIRS/NIB/FITO |
MIRS/National Institute of Biology/Department of Biotechnology and Systems Biology, Slovenia, EURAMET |
| NIM |
National Institute of Metrology, China, APMP |
| NMIA |
National Measurement Institute, Australia, Australia, APMP |
| UME |
TÜBITAK Ulusal Metroloji Enstitüsü, Türkiye, EURAMET |
| VNIIM |
D.I. Mendeleyev Institute for Metrology, Rosstandart, Russian Federation, COOMET |
This page proposes print-out on A4 paper (portrait) of the comparison details (best printed out using a black and white printer).
Please, select items to be printed out, then click on "OK" :
