Comparison Display

CCQM-K86.c

MEASURAND Copy number ratio DP-073494-4/FatA(A)

Degrees of equivalence represented by D_i = (x_i - x_R) and its expanded uncertainty U_i (k = 2), both expressed in cp/cp (%).

Sample T1: DP-073494-4/FatA(A)

D_i/ [cp/cp (%)]

Participating institutes

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CCQM-K86.c

MEASURAND Copy number ratio RT73/FatA

Degrees of equivalence represented by D_i = (x_i - x_R) and its expanded uncertainty U_i (k = 2), both expressed in cp/cp (%).

Sample T2: RT73/FatA

D_i/ [cp/cp (%)]

Participating institutes

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CQM-K86.c

MEASURAND Copy number ratio DP-073494-4/FatA(A)

Degrees of equivalence represented by D_i = (x_i - x_R) and its expanded uncertainty U_i (k = 2), both expressed in cp/cp (%).

LAB_i	D_i	U_neg,i	U_pos,i
	cp/cp (%)	cp/cp (%)	cp/cp (%)
CENAM	0.025	0.084
GL	0.025	0.048
INM	0.005	0.049
JRC	-0.015	0.026
LGC	0.005	0.03
NIB	0.015	0.098
NIM	0.045	0.026
NIMT	-0.013	0.018
NMIA	-0.01	0.022
NMIJ	-0.005	0.023
UME	-0.01	0.022
VNIIM	-0.005	0.031

Participating institutes

CCQM-K86.c

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CCQM-K86.c

MEASURAND Copy number ratio RT73/FatA

Degrees of equivalence represented by D_i = (x_i - x_R) and its expanded uncertainty U_i (k = 2), both expressed in cp/cp (%).

LAB_i	D_i	U_neg,i	U_pos,i
	cp/cp (%)	cp/cp (%)	cp/cp (%)
CENAM	-0.01	0.52
GLHK	0.49	0.94
INM	0.02	0.56
IRMM	-0.91	0.51
LGC	-0.31	0.48
NIB	1.73	0.58
NIM	1.2	1.18
NIMT	-0.99	0.47
NMIA	0.16	0.47
NMIJ	0	0.57
TUBITAK	2.32	0.71
VNIIM	2.62	1.35

Participating institutes

CCQM-K86.c

Comparison

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CCQM-K86.c

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Information & contact
Participants

Metrology area, Sub-field	Chemistry and Biology, Nucleic Acid Analysis
Description	Relative quantification of genomic DNA fragments extracted from a high oil matrix
Time of measurements	2016 - 2017
Status	Approved for equivalence

Final Reports of the comparisons	Metrologia 2020 57 Tech. Suppl. 08004
Measurand	Ratio of the numbers of copies of specified intact sequence fragments of a length up to 150 nucleotides following extraction from an unprocessed, high fat/oil ground seed matrix, with a copy number ratio from 0.001 to 1
Transfer device	Sample T1: Copy number ratio DP-073494-4/FatA(A) in genetically modified Brassica napus L (canola), expressed as a percentage. Sample T2: Copy number ratio RT73/FatA in genetically modified Brassica napus L (canola), expressed as a percentage.

Comparison type	Key Comparison
Consultative Committee	CCQM (Consultative Committee for Amount of Substance)
Conducted by	CCQM (Consultative Committee for Amount of Substance)
Comments	CCQM-K86.c results published on 16 January 2020
Pilot institute	LGC Laboratory of the Government Chemist United Kingdom
Contact person	Stephen ELLISON
Additional	Zoltan Mester, National Research Council Philippe Corbisier, Joint Research Centre

Pilot laboratory
LGC	Laboratory of the Government Chemist, United Kingdom, EURAMET
Note	LGC was coordinating laboratory for the purpose of report submission and co-authored the report, and the measurement campaign was coordinated jointly by JRC and NRC Canada

Pilot laboratory

LGC

Laboratory of the Government Chemist, United Kingdom, EURAMET

Note

LGC was coordinating laboratory for the purpose of report submission and co-authored the report, and the measurement campaign was coordinated jointly by JRC and NRC Canada

CENAM

Centro Nacional de Metrologia, Mexico, SIM

GL	Government Laboratory, Hong Kong, China, APMP

INM (CO)

Instituto Nacional de Metrología de Colombia, Colombia, SIM

JRC

Joint Research Centre, European Union, EURAMET

Pilot laboratory

The measurement campaign was coordinated jointly by JRC and NRC Canada

MIRS/NIB/FITO

MIRS/National Institute of Biology/Department of Biotechnology and Systems Biology, Slovenia, EURAMET

NIM	National Institute of Metrology, China, APMP

NIMT	National Institute of Metrology (Thailand), Thailand, APMP

NMIA	National Measurement Institute, Australia, Australia, APMP

NMIJ AIST

National Metrology Institute of Japan, Japan, APMP

NRC

National Research Council, Canada, SIM

Pilot laboratory

The measurement campaign was coordinated jointly by JRC and NRC Canada

UME	TÜBITAK Ulusal Metroloji Enstitüsü, Türkiye, EURAMET

VNIIM

D.I. Mendeleyev Institute for Metrology, Rosstandart, Russian Federation, COOMET

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CCQM-K86.c

MEASURAND Copy number ratio DP-073494-4/FatA(A)

The key comparison reference value x_R and its associated standard uncertainty u_R are obtained from, respectively, the median and median absolute deviation of the results used in the calculation, and both given as copy number ratio expressed as a percentage. All CCQM-K86.c participants’ results were used to calculate the key comparison reference value for Sample T1. The standard uncertainty for the median includes a scaling factor of sqrt(PI/2) for the asymptotic variance of the median, and additionally includes an allowance for inhomogeneity among samples provided to participants.

x_R = 0.115 cp/cp (%) u_R = 0.0086 cp/cp (%)

The degree of equivalence of laboratory i relative to the key comparison reference value x_R is given by a pair of terms, both expressed in cp/cp (%):

D_i = (x_i - x_R) and its expanded uncertainty U_i at a 95 % level of confidence where U_i = 2 u_i.

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CCQM-K86.c

MEASURAND Copy number ratio RT73/FatA

The key comparison reference value x_R and its associated standard uncertainty u_R are obtained from, respectively, the median and median absolute deviation of the results used in the calculation, and both given as copy number ratio expressed as a percentage. Only those CCQM-K86.c participants using FatA as reference gene were included in the calculation, participants using FatA(A) were not included. The standard uncertainty for the median includes a scaling factor of sqrt(PI/2) for the asymptotic variance of the median, and additionally includes an allowance for inhomogeneity among samples provided to participants.

x_R = 2.91 cp/cp (%) u_R = 0.22 cp/cp (%)

The degree of equivalence of laboratory i relative to the key comparison reference value x_R is given by a pair of terms, both expressed in cp/cp (%):

D_i = (x_i - x_R) and its expanded uncertainty U_i at a 95 % level of confidence where U_i = 2 u_i.

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CCQM-K86.c

MEASURAND Copy number ratio DP-073494-4/FatA(A)

x_i measurement value reported by participating laboratory i, Lab_i

u_i combined standard uncertainty of x_i

U_i expanded uncertainty of xi at 95 % level of confidence where U_i = 2u_i

Lab_i	x_i	u_i	U_i
	cp/cp (%)	cp/cp (%)	cp/cp (%)
JRC	0.100	0.009	0.03
NIMT	0.102	0.003	0.007
NMIA	0.105	0.007	0.015
UME	0.105	0.007	0.015
NMIJ	0.110	0.008	0.015
VNIIM	0.110	0.013	0.026
LGC	0.120	0.013	0.04
INM	0.120	0.023	0.046
NIB	0.130	0.048	0.096
CENAM	0.140	0.041	0.08204
GL	0.140	0.023	0.045
NIM	0.160	0.010	0.020

Note 1: Results are given as copy number ratio expressed as a percentage

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CQM-K86.c

MEASURAND Copy number ratio RT73/FatA

x_i measurement value reported by participating laboratory i, Lab_i

u_i combined standard uncertainty of x_i

U_i expanded uncertainty of xi at 95 % level of confidence where U_i = 2u_i

Lab_i	x_i	u_i	U_i
	cp/cp (%)	cp/cp (%)	cp/cp (%)
NIMT	1.918	0.077	0.155
JRC	2.000	0.132	0.42
LGC	2.600	0.094	0.3
CENAM	2.900	0.139	0.2784
NMIJ	2.910	0.185	0.37
INM	2.930	0.170	0.34
NMIA	3.060	0.088	0.21
GL	3.390	0.415	0.83
NIM *	4.100	0.550	1.1
NIB *	4.630	0.190	0.38
UME *	5.220	0.279	0.63
VNIIM *	5.520	0.640	1.280

Note 1: Results are given as copy number ratio expressed as a percentage.

Note 2 Four laboratories (marked *) used fatA(A) as reference gene.

KCDB

CIPM MRA

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Key and supplementary comparisons - Print out